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hegf  (R&D Systems)


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  • 97

    Structured Review

    R&D Systems hegf
    Hegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hegf/Recombinant+Human+EGF+Protein%2C+CF/us12605397-692-57-58
    Average 97 stars, based on 1262 article reviews
    hegf - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Knock-Out:

    Article Title: X‐Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome
    Article Snippet: .. The PGCLC medium is 15% Knockout serum replacement (Gibco, 10828‐028) Glasgow's MEM medium (Gibco, 11710‐035) with addition of non‐essential amino acids solution (Gibco,11140‐050), sodium pyruvate (Gibco,11360‐070), Penicillin‐streptomycin‐Glutamine (Gibco, 10378‐016), 2‐Mercaptoethanol (Gibco, 21985‐023), animal‐free recombinant Activin A (Pepro tech, AF‐120‐14E), and cytokine including hLIF (Stem cell, 78 055), hEGF (R&D systems, 236‐EG‐200), hSCF (Peprotech, 300–07), BMP4 (R&D systems, 314‐BP‐500), Y‐27632 (Dihydrochloride) ROCK pathway inhibitor (Stem cell technologies,72 307). ..

    Article Title: X-Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome.
    Article Snippet: .. The PGCLC medium is 15% Knockout serum replacement (Gibco, 10828-028) Glasgow’s MEM medium (Gibco, 11710-035) with addition of non-essential amino acids solution (Gibco,11140-050), sodium pyruvate (Gibco,11360-070), Penicillinstreptomycin-Glutamine (Gibco, 10378-016), 2-Mercaptoethanol (Gibco, 21985-023), animal-free recombinant Activin A (Pepro tech, AF-120-14E), and cytokine including hLIF (Stem cell, 78 055), hEGF (R&D systems, 236- EG-200), hSCF (Peprotech, 300–07), BMP4 (R&D systems, 314-BP-500), Y-27632 (Dihydrochloride) ROCK pathway inhibitor (Stem cell technologies,72 307). ..

    Recombinant:

    Article Title: X‐Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome
    Article Snippet: .. The PGCLC medium is 15% Knockout serum replacement (Gibco, 10828‐028) Glasgow's MEM medium (Gibco, 11710‐035) with addition of non‐essential amino acids solution (Gibco,11140‐050), sodium pyruvate (Gibco,11360‐070), Penicillin‐streptomycin‐Glutamine (Gibco, 10378‐016), 2‐Mercaptoethanol (Gibco, 21985‐023), animal‐free recombinant Activin A (Pepro tech, AF‐120‐14E), and cytokine including hLIF (Stem cell, 78 055), hEGF (R&D systems, 236‐EG‐200), hSCF (Peprotech, 300–07), BMP4 (R&D systems, 314‐BP‐500), Y‐27632 (Dihydrochloride) ROCK pathway inhibitor (Stem cell technologies,72 307). ..

    Article Title: X-Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome.
    Article Snippet: .. The PGCLC medium is 15% Knockout serum replacement (Gibco, 10828-028) Glasgow’s MEM medium (Gibco, 11710-035) with addition of non-essential amino acids solution (Gibco,11140-050), sodium pyruvate (Gibco,11360-070), Penicillinstreptomycin-Glutamine (Gibco, 10378-016), 2-Mercaptoethanol (Gibco, 21985-023), animal-free recombinant Activin A (Pepro tech, AF-120-14E), and cytokine including hLIF (Stem cell, 78 055), hEGF (R&D systems, 236- EG-200), hSCF (Peprotech, 300–07), BMP4 (R&D systems, 314-BP-500), Y-27632 (Dihydrochloride) ROCK pathway inhibitor (Stem cell technologies,72 307). ..

    Centrifugation:

    Article Title: Improving in vitro induction efficiency of human primordial germ cell-like cells using N2B27 or NAC-based medium
    Article Snippet: The N2B27 medium contained Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM-F12; Cat. #11330032, Thermo Fisher Scientific) supplemented with N2 (Cat. #17502001, Thermo Fisher Scientific), B27 (Cat. #17504044, Thermo Fisher Scientific), 0.1 mmol/L NEAA, and 0.1 mmol/L 2-mercaptoethanol. hPSCs were treated with TrypLE Express (Cat. #12605028, Gibco) at 37 °C for 3 min, followed by gentle mechanical dissociation using a pipette. .. After centrifugation, the cell pellets were resuspended in GK15 or N2B27 media, supplemented with 200 ng/mL hBMP4 (Cat. #314-BP, R&D Systems), 100 ng/mL hSCF (Cat. #BT-SCF, R&D Systems), 50 ng/mL hEGF (Cat. #236-EG, R&D Systems), 1000 U/mL hLIF (Cat. #7734-LF, R&D Systems), and 10 μmol/L of Y-27632 to induce hPGCLCs. .. Next, 1.5 × 10 6 cells were mixed with 2 mL of culture medium and seeded into one well of the 12-well AggreWell TM 800 culture plate (Cat. #43815, Stem Cell Technologies, Vancouver, BC, Canada) pretreated with an anti-adherence rinsing solution (Cat. #07010, Stem Cell Technologies) following the manufacturer's instructions.



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